Reaktion #345322

ord-b6b2a7cdd38d472a9e46f5d143d3e42e

Reaktionsgleichung

O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES
O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES
[F-].[K+]
KF
O[C@H](CS)[C@H](O)CS
DTT
[F-].[K+]
Potassium fluoride
→
CC(C)=CCC/C(C)=C/CC/C(C)=C/CC/C=C(\C)CC/C=C(\C)CCC=C(C)C
Squalene

Edukte

O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES
O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES
—
Dithiothreitol(DTT)
—
mixture
[F-].[K+]
KF
O[C@H](CS)[C@H](O)CS
DTT
[F-].[K+]
Potassium fluoride

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

Buffer mixture contains 270 mM HEPES, pH 7.5, 20 mM Potassium fluoride and 5.4 mM Dithiothreitol(DTT). 55 μl of this mixture was used per assay. The final concentrations of HEPES, KF and DTT in the assay are 150 mM, 11 mM and 3 mM respectively.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05264593uspto-grants-1993_11