Reaktion #340408

Reaktionsgleichung

2-[3,4-bis(methyloxy)phenyl]-4-(methyloxy)-3-oxobutanenitrile

hydrazine dihydrochloride

3-chloro-4-hydroxybenzaldehyde

trifluoroacetic acid

sodium bicarbonate

→

4-{7,8-bis(methyloxy)-1-[(methyloxy)methyl]-3H-pyrazolo[3,4-c]isoquinolin-5-yl}-2-chlorophenol hydrochloride salt

Ausbeute 38.3%

Lösungsmittel

ethanol

methanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   TemperaturThe resulting mixture was heated
2. 2
   Temperaturat reflux for 1 hour
3. 3
   TemperaturIt was cooled
4. 4
   Extraktionthe mixture was extracted with ethyl acetate (3×10 mL)
5. 5
   ExtraktionThe combined extract
6. 6
   Waschenwas washed with water and brine (30 mL each)
7. 7
   Trocknendried over sodium sulfate
8. 8
   Filtrationfiltered
9. 9
   Einengenconcentrated
10. 10
    Sonstigeto give oily residue
11. 11
    EinengenThen the reaction mixture was concentrated
12. 12
    workup.DISSOLUTIONdissolved in 10 mL of N,N-dimethylformamide
13. 13
    Sonstigepurified by preparatory reverse phase HPLC (Shimadzu LC-8A HPLC, Waters Xterra C18 30 mm×100 mm column, acetonitrile-water-trifluoroacetic buffer)
14. 14
    SonstigeAfter lyophillization of the pure fractions a yellow powder was obtained
15. 15
    Sonstige4N hydrogen chloride in dioxane, and evaporated to dryness (repeated three times)

Vorschrift

To a solution of 2-[3,4-bis(methyloxy)phenyl]-4-(methyloxy)-3-oxobutanenitrile (200 mg, 0.8 mmol) in ethanol (5 mL) was added hydrazine dihydrochloride (101 mg, 0.9 mmol). The resulting mixture was heated at reflux for 1 hour. It was cooled, pour into a saturated sodium bicarbonate solution (10 mL), and the mixture was extracted with ethyl acetate (3×10 mL). The combined extract was washed with water and brine (30 mL each), dried over sodium sulfate, filtered and concentrated to give oily residue. To this residue was added of 3-chloro-4-hydroxybenzaldehyde (189 mg, 1.2 mmol) and trifluoroacetic acid (3 mL), and the resulting mixture was heated at 70° C. for 18 hours. Then the reaction mixture was concentrated, dissolved in 10 mL of N,N-dimethylformamide and purified by preparatory reverse phase HPLC (Shimadzu LC-8A HPLC, Waters Xterra C18 30 mm×100 mm column, acetonitrile-water-trifluoroacetic buffer). After lyophillization of the pure fractions a yellow powder was obtained. The solid was dissolved in 4 mL of 1:1 methanol: 4N hydrogen chloride in dioxane, and evaporated to dryness (repeated three times) to give 4-{7,8-bis(methyloxy)-1-[(methyloxy)methyl]-3H-pyrazolo[3,4-c]isoquinolin-5-yl}-2-chlorophenol hydrochloride salt (101 mg, 0.23 mmol, 29% yield). 1H NMR (400 MHz, d6-DMSO): 10.64 (b, 1H), 7.82 (s, 1H), 7.71 (s, 1H), 7.58-7.56 (d, 1H), 7.45 (s, 1H), 7.17-7.15 (d, 1H), 4.92 (s, 2H), 4.02 (s, 3H), 3.78 (s, 3H); MS (EI) for C20H18ClN3O4: 400 (MH+).

Quelle