Reaktion #338555

Reaktionsgleichung

5-amino-1-(5-cyclopropylcarbamoyl-2-methylphenyl)-1H-pyrazole-4-carboxylic acid

2-methylcyclohexylamine

EDCI

HOBt

→

5-amino-1-(5-cyclopropylcarbamoyl-2-methylphenyl)-1H-pyrazole-4-carboxylic acid (2-methylcyclohexyl)-amide

Ausbeute 70.0%

Lösungsmittel

Water

DMF

Reaktionsbedingungen

Aufarbeitung

1. 1
   Extraktionthe solution was extracted with ethyl acetate (3 mL ×2)
2. 2
   WaschenThe organic phase was then washed with water (3 mL)
3. 3
   Trocknendried over Na2SO4
4. 4
   Sonstigeevaporated
5. 5
   SonstigeThe residue was purified by preparative TLC plate

Vorschrift

A solution of 5-amino-1-(5-cyclopropylcarbamoyl-2-methylphenyl)-1H-pyrazole-4-carboxylic acid (21 mg), 2-methylcyclohexylamine (10 mg), EDCI (28 mg) and HOBt (12 mg) in DMF (0.75 ml) was reacted at room temperature for 24 h. Water (4 ml) was added and the solution was extracted with ethyl acetate (3 mL ×2). The organic phase was then washed with water (3 mL), dried over Na2SO4 and evaporated. The residue was purified by preparative TLC plate to give the desired product 5-amino-1-(5-cyclopropylcarbamoyl-2-methylphenyl)-1H-pyrazole-4-carboxylic acid (2-methylcyclohexyl)-amide (19 mg, 70%). HPLC (4 minute gradient) tR 2.34 min; MS m/z 396.31 [M+H]+.

Quelle