Reaktion #334190

ord-ef59350ea5c44018ad6e72e58e663dac

Reaktionsgleichung

NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NADH
CC(O)C(=O)[O-]
lactate
→
CC(O)C(=O)[O-]
lactate
NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)c1
NAD+

Edukte

NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
NADH
CC(O)C(=O)[O-]
lactate

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

In the case of this determination, ADP is reacted with phosphoenol pyruvate (PEP) in the presence of pyruvate kinase (PK) to give pyruvate which, in turn, is reacted with NADH in the presence of lactate dehydrogenase (LDH) to give lactate and NAD+.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04705749uspto-grants-1987_11