Reaktion #321772

ord-6b3c35f6f848441c8a2ccfd049611ed2

Reaktionsgleichung

CC(=O)[O-]
acetate
O=C([O-])O.O=C([O-])[O-]
carbonate bicarbonate
CC(=O)[O-].CCC1(CC)C(=O)NC(=O)NC1=O.Cl.[Na+]
Veronal sodium acetate-HCl
NCC(=O)O.[Na+].[OH-]
glycine NaOH
[O-]B([O-])[O-]
borate
O=P([O-])([O-])[O-]
phosphate
→
O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA

Edukte

CC(=O)[O-]
acetate
—
TRIS-HCl
O=C([O-])O.O=C([O-])[O-]
carbonate bicarbonate
CC(=O)[O-].CCC1(CC)C(=O)NC(=O)NC1=O.Cl.[Na+]
Veronal sodium acetate-HCl
NCC(=O)O.[Na+].[OH-]
glycine NaOH
[O-]B([O-])[O-]
borate
O=P([O-])([O-])[O-]
phosphate

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    SonstigeAny buffer providing the desired pH which
  2. 2
    Sonstigeto provide a pH of 6.0 or less
  3. 3
    Sonstigeto provide a pH of from 5.5 to 9.5
  4. 4
    Sonstigeto provide a pH of from 8.5 to 11.0
  5. 5
    Sonstigeto provide a pH of from 5.0 to 9.0
  6. 6
    Sonstigeto provide a pH of from 3.0 to 9.0
  7. 7
    Sonstigeto provide a pH of from 6.8 to 8.5
  8. 8
    Sonstigeto provide a pH of from 8.5 to 9.5
  9. 9
    Sonstigeto provide a pH of from 8.0 to 11.0

Vorschrift

Any buffer providing the desired pH which does not interfere the measurement of enzyme activity is suitable for use in the present invention. However, more specifically, a conventional acetate buffer can be used to provide a pH of 6.0 or less; a conventional phosphate buffer can be used to provide a pH of from 5.5 to 9.5; a carbonate-bicarbonate buffer (e.g., K2CO3 --NaHCO3) buffer can be used to provide a pH of from 8.5 to 11.0; a conventional Britton-Robinson buffer can be used to provide a pH of from 5.0 to 9.0; a conventional Veronal-sodium acetate-HCl buffer can be used to provide a pH of from 3.0 to 9.0; a TRIS-HCl buffer can be used to provide a pH of from 6.8 to 8.5; a conventional glycine-NaOH buffer can be used to provide a pH of from 8.5 to 9.5; a conventional borate buffer can be used to provide a pH of from 8.0 to 11.0; a conventional EDTA buffer can provide a pH of about 8.5; etc.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05595867uspto-grants-1997_01