Reaktion #312087

Reaktionsgleichung

dipotassium hydrogen phosphate

2-(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetic acid

chloroformic acid isobutyl ester

3-acetoxymethyl-7β-amino-3-cephem-4-carboxylic acid tert.-butyl ester

→

3-acetoxymethyl-7β-[2-(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetylamino]-3-cephem-4-carboxylic acid tert.-butyl ester

Lösungsmittel

methylene chloride

N-methylmorpholine

methylene chloride

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe starting material can be prepared
2. 2
   Sonstigeat between -15° C. and -20° C
3. 3
   Temperaturslowly raising the temperature to 20° C. to 25° C
4. 4
   workup.ADDITIONThe mixture is poured out into ice-cold water
5. 5
   Extraktionextracted repeatedly with methylene chloride
6. 6
   WaschenThe organic extracts are washed with an aqueous saturated sodium chloride solution
7. 7
   Trocknendried over magnesium sulphate
8. 8
   Sonstigeafter which the solvent is removed under reduced pressure
9. 9
   SonstigeThe residue is recrystallised from diethyl ether

Vorschrift

The starting material can be prepared as follows: 8.15 g of 2-(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetic acid are dissolved in 500 ml of methylene chloride containing 3.06 ml of N-methylmorpholine, the solution, from which moisture is kept excluded, is cooled to -20° C. and 4.13 ml of chloroformic acid isobutyl ester are added dropwise whilst keeping the temperature at between -15° C. and -20° C. After 30 minutes, a solution of 9.85 g of 3-acetoxymethyl-7β-amino-3-cephem-4-carboxylic acid tert.-butyl ester in 50 ml of methylene chloride is added, after which the solution is stirred for a further 15 hours whilst slowly raising the temperature to 20° C. to 25° C. The mixture is poured out into ice-cold water, neutralised with dipotassium hydrogen phosphate and extracted repeatedly with methylene chloride. The organic extracts are washed with an aqueous saturated sodium chloride solution and dried over magnesium sulphate, after which the solvent is removed under reduced pressure. The residue is recrystallised from diethyl ether and gives 3-acetoxymethyl-7β-[2-(5-tert.-butoxycarbonylaminomethyl-2-thienyl)-acetylamino]-3-cephem-4-carboxylic acid tert.-butyl ester, melting point 76°-78° C., thin layer chromatogram (silica gel): Rf=0.44 (system: chloroform/ethyl acetate/acetic acid, 80:19:1).

Quelle