Reaktion #310540

Reaktionsgleichung

ethylenediamine tetraacetic acid

ascorbic acid

2-(2-methoxy-4-methylsulfinyl-phenyl)-1H-imidazo[4,5-b]pyridine

→

2-(2-Methoxy-4-methylsulfinyl-phenyl)-6-hydroxy-1H-imidazo[4,5-b]pyridine

Lösungsmittel

phosphate

phosphate

hydrogen peroxide

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeAfter 30 minutes the reaction mixture was evaporated in vacuo
2. 2
   Sonstigethe residue was purified by chromatography on a polystyrene resin column (XAD-2 of Rohm & Haas) with water and methanol as eluants
3. 3
   SonstigeAfter evaporation of the eluate the two compounds
4. 4
   Sonstigewere separated by means of chromatography on silicagel [eluant: chloroform/methanol/ammonia water=85/15/2 (v/v)]

Vorschrift

7.2 gm of 2-(2-methoxy-4-methylsulfinyl-phenyl)-1H-imidazo[4,5-b]pyridine were dissolved in a mixture of a phosphate buffer of pH 7.2 and 120 ml of 3% hydrogen peroxide, and a solution of 9.3 gm of ethylenediamine tetraacetic acid, 4.4 gm of ascorbic acid, 3.8 gm of iron(II) sulfate in a phosphate buffer of pH 7.2 was added. After 30 minutes the reaction mixture was evaporated in vacuo, and the residue was purified by chromatography on a polystyrene resin column (XAD-2 of Rohm & Haas) with water and methanol as eluants. After evaporation of the eluate the two compounds were separated by means of chromatography on silicagel [eluant: chloroform/methanol/ammonia water=85/15/2 (v/v)]. 2-(2-Methoxy-4-methylsulfinyl-phenyl)-6-hydroxy-1H-imidazo[4,5-b]pyridine was obtained as yellow powder.

Quelle