Reaktion #306455

Reaktionsgleichung

6-((6-iodo-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl)-3-methoxyquinoline

5-bromo-3-methylisothiazole

isopropylmagnesium chloride

4,5-bis(diphenylphosphino)-9,9-dimethyl-9H-xanthene

→

3-methoxy-6-((6-(3-methylisothiazol-5-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl)quinoline

Ausbeute 1.6%

Lösungsmittel

THF

THF

THF

THF

Reaktionsbedingungen

Aufarbeitung

1. 1
   Temperaturto warm to rt
2. 2
   workup.STIRRINGThe mixture was stirred at rt 10 minutes
3. 3
   workup.STIRRINGthe slurry stirred 10 minutes
4. 4
   SonstigeThe flask was fitted with a reflux condenser
5. 5
   Temperaturheated with a 90° C. oil bath for 24 h
6. 6
   workup.ADDITIONadded to the reaction mixture
7. 7
   Temperaturthe mixture heated at 90° C. for 1 h
8. 8
   Temperaturto cool to rt
9. 9
   Wascheneluted with 200 mL THF
10. 10
    EinengenThe eluents were concentrated in vacuo
11. 11
    workup.STIRRINGthe mixture stirred at room temperature for 24 h
12. 12
    Sonstigethe solid collected
13. 13
    WaschenThe solid was washed with EtOH (discarded)
14. 14
    EinengenThe ammoniacal eluents were concentrated
15. 15
    Sonstigethe residue purified by HPLC

Vorschrift

A flask charged with 5-bromo-3-methylisothiazole (0.049 g, 0.28 mmol) and 0.3 mL dry THF was cooled to 0° C., and isopropylmagnesium chloride (0.30 ml, 0.30 mmol) added dropwise over 5 minutes and the mixture stirred an additional 5 minutes before allowing to warm to rt. The mixture was stirred at rt 10 minutes and the mixture cannulated to a solution of zinc(II) chloride (0.30 ml, 0.30 mmol) [1M] and the slurry stirred 10 minutes. The zincate was treated with 6-((6-iodo-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl)-3-methoxyquinoline (0.0890 g, 0.21 mmol), 1 mL dry THF, and a preformed solution of 4,5-bis(diphenylphosphino)-9,9-dimethyl-9H-xanthene (0.012 g, 0.021 mmol) and Pd(OAc)2 (0.0024 g, 0.011 mmol) dissolved in 1 mL dry THF. The flask was fitted with a reflux condenser and heated with a 90° C. oil bath for 24 h. The zincate portion of this procedure was repeated, added to the reaction mixture, and the mixture heated at 90° C. for 1 h. The mixture was allowed to cool to rt and loaded onto 10 g of SiO2 wet-packed with THF, and eluted with 200 mL THF. The eluents were concentrated in vacuo, and taken up in 5 mL EtOH. To the solution was added Si-sulfonic acid (1.5 g, 1.1 mmol) and the mixture stirred at room temperature for 24 h, and the solid collected. The solid was washed with EtOH (discarded), and then 2M NH3 in EtOH (5×5 mL). The ammoniacal eluents were concentrated and the residue purified by HPLC to afford 3-methoxy-6-((6-(3-methylisothiazol-5-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methyl)quinoline (0.0013 g, 1.6% yield).

Quelle