Reaktion #303942

Reaktionsgleichung

carbontetrabromide

(8-methyl-2-o-tolylquinolin-3-yl)methanol

triphenylphosphine

HCl

sodium hydroxide

3-(bromomethyl)-8-methyl-2-o-tolylquinoline

6-mercaptopurine monohydrate

→

3-((9H-Purin-6-ylthio)methyl)-8-methyl-2-o-tolylquinoline

Lösungsmittel

brine

CH2Cl2

THF

Reaktionsbedingungen

Aufarbeitung

1. 1
   EinengenThe crude mixture was concentrated under reduced pressure
2. 2
   Sonstigeevaporated onto silica gel
3. 3
   Sonstigepurified by flash chromatography (Biotage® Si 25+M)
4. 4
   Wascheneluting with EtOAc/hexane (0% to 10%)
5. 5
   Sonstigeto provide an off-white solid
6. 6
   Sonstigeused without further purification, MS (ESI+) m/z=326.0 (M)
7. 7
   Temperaturthe biphasic mixture was heated
8. 8
   Temperaturunder reflux for 5 h
9. 9
   TemperaturThe reaction mixture was cooled to 0° C.
10. 10
    Extraktionextracted with THF (3×15 mL)
11. 11
    TrocknenThe combined organic layers were dried (MgSO4)
12. 12
    Einengenconcentrated under reduced pressure
13. 13
    workup.DISSOLUTIONThe resulting yellow solid was dissolved in THF/DMSO
14. 14
    Sonstigeevaporated onto silica gel
15. 15
    Sonstigepurified by flash chromatography (Biotage® Si 25+M)
16. 16
    Wascheneluting with acetone/hexane (20% to 50%)
17. 17
    SonstigeThe resulting off-white solid was recrystallized from THF/MeOH
18. 18
    Sonstigeto provide a white solid [PI3δ IC50=889 nM]

Vorschrift

Solid carbontetrabromide (429 mg, 1.29 mmol) was added to a mixture of (8-methyl-2-o-tolylquinolin-3-yl)methanol (227 mg, 0.86 mmol) and triphenylphosphine (339 mg, 1.29 mmol) in CH2Cl2 (5 mL) at 0° C., and the mixture was stirred at 0° C. for 0.5 h. The crude mixture was concentrated under reduced pressure, evaporated onto silica gel, and purified by flash chromatography (Biotage® Si 25+M) eluting with EtOAc/hexane (0% to 10%) to provide an off-white solid; used without further purification, MS (ESI+) m/z=326.0 (M). A 2.0M aqueous solution of sodium hydroxide (0.86 mL, 1.72 mmol) was added to a mixture of 3-(bromomethyl)-8-methyl-2-o-tolylquinoline (140 mg, 0.43 mmol) and 6-mercaptopurine monohydrate (146 mg, 0.86 mmol) in THF (1.6 mL), and the biphasic mixture was heated under reflux for 5 h. The reaction mixture was cooled to 0° C., neutralized by the addition of 1N aqueous HCl, diluted with brine (10 mL), and extracted with THF (3×15 mL). The combined organic layers were dried (MgSO4) and concentrated under reduced pressure. The resulting yellow solid was dissolved in THF/DMSO, evaporated onto silica gel, and purified by flash chromatography (Biotage® Si 25+M) eluting with acetone/hexane (20% to 50%). The resulting off-white solid was recrystallized from THF/MeOH to provide a white solid [PI3δ IC50=889 nM]. MS (ESI+) m/z=398.1 (M+1).

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