Reaktion #2979

Reaktionsgleichung

(2S, 4R)-N-t-butoxycarbonyl- 4-methyl glutamic acid

→

(2S, 4R)-4-Methyl Glutamic Acid

Lösungsmittel

trifluoroacetic acid methylene chloride

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe volatiles were removed in vacuo
2. 2
   Sonstigethe residue was azeotroped with toluene (50 mL)
3. 3
   workup.ADDITIONWater (150 mL) was added
4. 4
   Extraktionthe aqueous phase extracted with a 5% solution of trioctylamine in chloroform (3 x 200 mL)
5. 5
   WaschenThe combined organic phases were washed with water (50 mL)
6. 6
   Sonstigethe combined aqueous phases evaporated
7. 7
   Sonstigeto give the product (4.6 g, 66% in two steps) as a white foam which
8. 8
   Sonstigewas recrystallized in acetone

Vorschrift

The Boc-protected diacid, (2S, 4R)-N-t-butoxycarbonyl- 4-methyl glutamic acid, was subjected to a mixture of trifluoroacetic acid:methylene chloride (40:60, 100 mL) for 3 hr at room temperature. The volatiles were removed in vacuo and the residue was azeotroped with toluene (50 mL). Water (150 mL) was added and the aqueous phase extracted with a 5% solution of trioctylamine in chloroform (3 x 200 mL). The combined organic phases were washed with water (50 mL) and the combined aqueous phases evaporated by being placed on a lyophilizer for 48 hr to give the product (4.6 g, 66% in two steps) as a white foam which was recrystallized in acetone and water. mp=169°-170° C., 1H NMR (200 MHz, D2O): δ 3.80 (dd, J=7.3, 7.3 Hz, 1H), 2.55 (m, 1H), 2.15 (ddd, J=14.7, 8.7, 6.6 Hz, 1H), 1.75 (ddd, J=14.6, 7.4, 5.4 Hz, 1H), 1.05 (d, J=7.0 Hz, 3H). Anal. Calculated for C6H11NO4 : C, 44.71; H, 6.88; N, 8.69. Found: C, 44.59; H, 6.85; N, 8.61.

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