Reaktion #2507845

ord-6c8e633799a94f37a39153d5f8c3caac

Reaktionsgleichung

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl
→
OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
Trehalose

Edukte

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl

Reagenzien

Keine

Lösungsmittel

O
water
O
water

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONwas added at room temperature
  2. 2
    Sonstigewas obtained
  3. 3
    Sonstigeto obtain 400 ml final volume
  4. 4
    FiltrationThe solution was filtered through a 0.22-micron
  5. 5
    Filtrationfilter
  6. 6
    workup.ADDITIONto dilute the protein solution

Vorschrift

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07956028B2uspto-grants-2011_06