Reaktion #2366329

Reaktionsgleichung

(2-{4-[6-(4-Methoxymethoxy-phenyl)-3-methyl-1-(tetrahydro-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-ylmethyl]-2,2,5,5-tetramethyl-piperazin-1-yl}-ethyl)-methyl-carbamic acid tert-butyl ester

hydrochloric acid

→

title compound

Ausbeute 5.2%

4-{3-Methyl-4-[2,2,5,5-tetramethyl-4-(2-methylamino-ethyl)-piperazin-1-ylmethyl]-1H-pyrazolo[3,4-b]pyridin-6-yl}-phenol

Ausbeute 5.2%

Lösungsmittel

1,4-dioxane

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigethe volatiles were removed in vacuo
2. 2
   SonstigeThe residue was purified by preparative HPLC (C18 column, acetonitrile/water gradient)

Vorschrift

1320 mg of the crude (2-{4-[6-(4-Methoxymethoxy-phenyl)-3-methyl-1-(tetrahydro-pyran-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-ylmethyl]-2,2,5,5-tetramethyl-piperazin-1-yl}-ethyl)-methyl-carbamic acid tert-butyl ester were dissolved in 6 ml of 1,4-dioxane and treated with 4 ml of hydrochloric acid (4M in dry dioxane). After standing at rt for 1.5 h the volatiles were removed in vacuo. The residue was purified by preparative HPLC (C18 column, acetonitrile/water gradient) and 45 mg (5%) of the title compound were obtained.

Quelle