Reaktion #2274431

Reaktionsgleichung

phosphate

racemic ethyl(1SR,5RS,6SR)-2-oxobicyclo [3.1.0]hexane-6-carboxylate

→

(+)-(1S,5R, 6S)-2-oxobicyclo[3.1.0]hexane-6-carboxylic acid

Lösungsmittel

n-hexane

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.ADDITIONwas added in an amount
2. 2
   workup.DISSOLUTIONdissolved
3. 3
   workup.ADDITIONadded to the enzyme solution
4. 4
   Sonstigean aliquot of the reaction solution was removed
5. 5
   Sonstigethe optical purity and reaction yield of the formed 2-oxobicyclo[3.1.0]hexane-6-carboxylic acid

Vorschrift

Each of the various commercial enzymes shown in Table 3 was added in an amount shown in Table 4 to 1 ml of 100 mM phosphate buffer (pH 7.0) and dissolved therein. 10 mg of racemic ethyl(1SR,5RS,6SR)-2-oxobicyclo [3.1.0]hexane-6-carboxylate was dissolved in 1 ml n-hexane, then added to the enzyme solution, and heated to 40° C. and the mixture was stirred. After the time shown as the reaction time in Table 4 elapsed, an aliquot of the reaction solution was removed and analyzed in the same manner as in Examples 1 to 8 to determine the optical purity and reaction yield of the formed 2-oxobicyclo[3.1.0]hexane-6-carboxylic acid. (+)-(1S,5R, 6S)-2-oxobicyclo[3.1.0]hexane-6-carboxylic acid was obtained in excess. The results are shown in Table 4.

Quelle