Reaktion #215180

Reaktionsgleichung

N-acetyl-DL-methionine

→

L-methionine

Reaktionsbedingungen

Aufarbeitung

1. 1
   FiltrationThe complex is filtered

Vorschrift

The enzymatic activity of the complex is determined by contacting 500 mg. of complex with 50 ml. of an 0.4 weight percent solution of N-acetyl-DL-methionine in 0.1 M TRIS buffer, of pH 8, containing 5·10-4M of cobalt chloride, at 37° C., and agitated for 30 minutes. The complex is filtered and the L-methionine formed is determined in the filtrate.

Quelle