Reaktion #2128153

Reaktionsgleichung

(R)-6-bromo-2-(3-(piperidin-1-yl)pyrrolidin-1-yl)benzo[d]thiazole

pyridazin-3(2H)-one

potassium carbonate

N1,N2-dimethylethane-1,2-diamine

→

(R)-2-(2-(3-(piperidin-1-yl)pyrrolidin-1-yl)benzo[d]thiazol-6-yl)pyridazin-3(2H)-one

Lösungsmittel

diethyl ether

Pyridine

toluene

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigevial equipped with a magnetic stirbar
2. 2
   SonstigeThe vial was crimp capped with a septum
3. 3
   SonstigeThe reaction mixture was purged (vacuum/nitrogen) three times
4. 4
   Temperaturto cool to room temperature
5. 5
   Sonstigethe contents were transferred to a 100 mL round bottom flask
6. 6
   Waschen(methanol rinse)
7. 7
   SonstigeVolatiles were removed under reduced pressure
8. 8
   Sonstigethe residue was partitioned between CHCl3 and aqueous ammonium hydroxide
9. 9
   WaschenThe organic layer was washed with brine
10. 10
    Trocknenthen dried (MgSO4)
11. 11
    Filtrationfiltered
12. 12
    EinengenThe filtrate was concentrated under reduced pressure
13. 13
    Sonstigeto give a solid
14. 14
    Sonstigereturned to the rotary evaporator in an attempt
15. 15
    Sonstigeto remove residual pyridine
16. 16
    SonstigeA solid was obtained
17. 17
    SonstigeThis was purified by column chromatography on an Analogix IntelliFlash-280 (Analogix SF65-220 g, 100% CHCl3 to 97:3 CHCl3/methanol)
18. 18
    workup.ADDITIONFractions containing product
19. 19
    Einengenconcentrated under reduced pressure
20. 20
    Sonstigeto give a solid
21. 21
    FiltrationThe solid was collected by filtration
22. 22
    Waschenrinsed once with ethyl acetate and several times with hexane
23. 23
    SonstigeThis solid was crystallized from hot ethyl acetate
24. 24
    FiltrationThe first crystal batch was collected by filtration
25. 25
    Sonstigedried in a vacuum oven at 84° C. for 5 hours
26. 26
    EinengenThe filtrate was concentrated under reduced pressure
27. 27
    workup.DISSOLUTIONthe solid residue was dissolved in hot ethyl acetate in a second crystallization attempt
28. 28
    FiltrationThe second crystal batch was collected by filtration
29. 29
    Sonstigedried in the vacuum oven along with the first batch at 88° C. overnight

Vorschrift

A mixture of (R)-6-bromo-2-(3-(piperidin-1-yl)pyrrolidin-1-yl)benzo[d]thiazole (Example 14, 0.792 g, 2.162 mmol), pyridazin-3(2H)-one (0.415 g, 4.32 mmol), copper powder (0.137 g, 2.16 mmol), copper(I) iodide (57.6 mg, 0.303 mmol), and potassium carbonate (0.896 g, 6.49 mmol) were combined into a large Biotage microwave vial equipped with a magnetic stirbar. The vial was crimp capped with a septum. Pyridine (17.3 mL) was introduced via syringe. The reaction mixture was purged (vacuum/nitrogen) three times, then N1,N2-dimethylethane-1,2-diamine (0.065 mL, 0.605 mmol) was added via syringe and the reaction mixture was stirred with heating at 117° C. for 24 hours. The reaction mixture was allowed to cool to room temperature then the vial was uncapped and the contents were transferred to a 100 mL round bottom flask (methanol rinse). Volatiles were removed under reduced pressure and the residue was partitioned between CHCl3 and aqueous ammonium hydroxide. The organic layer was washed with brine then dried (MgSO4) and filtered. The filtrate was concentrated under reduced pressure to give a solid that was treated with toluene and returned to the rotary evaporator in an attempt to remove residual pyridine. A solid was obtained. This was purified by column chromatography on an Analogix IntelliFlash-280 (Analogix SF65-220 g, 100% CHCl3 to 97:3 CHCl3/methanol). Fractions containing product were combined and concentrated under reduced pressure to give a solid that was stirred with diethyl ether. The solid was collected by filtration and rinsed once with ethyl acetate and several times with hexane. This solid was crystallized from hot ethyl acetate. The first crystal batch was collected by filtration and dried in a vacuum oven at 84° C. for 5 hours. The filtrate was concentrated under reduced pressure and the solid residue was dissolved in hot ethyl acetate in a second crystallization attempt. The second crystal batch was collected by filtration and dried in the vacuum oven along with the first batch at 88° C. overnight. The two dried crystal batches combined gave (R)-2-(2-(3-(piperidin-1-yl)pyrrolidin-1-yl)benzo[d]thiazol-6-yl)pyridazin-3(2H)-one. 1H NMR (300 MHz, CD3OD) δ ppm 1.51-1.59 (m, 2H), 1.70 (pentet, J=6 Hz, 4H), 1.99-2.15 (m, 1H), 2.36-2.47 (m, 1H), 2.56-2.77 (m, 4H), 3.17-3.31 (m, 1H), 3.47 (t, J=9 Hz, 1H), 3.53-3.64 (m, 1H), 3.73-3.82 (m, 1H), 3.87-3.96 (m, 1H), 7.09 (dd, J=2, 9 Hz, 1H), 7.45-7.51 (m, 1H), 7.57 (d, J=9 Hz, 1H), 7.89 (d, J=2 Hz, 1H), 8.04 (dd, J=2, 4 Hz, 1H); MS (DCI/NH3) m/z 382 (M+H)+.

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