Reaktion #2045075

ord-a9ed993f607244468b26bd933c6df344

Reaktionsgleichung

[Na+].[OH-]
NaOH
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
OC[C@@H](O)[C@H](O)[C@@H](O)CO
xylitol

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONby dilute acid
  2. 2
    Sonstigeis 130° C.
  3. 3
    Sonstigeis controlled at 30° C.
  4. 4
    Temperaturthe agitation of the incubator is maintained at 100 rpm
  5. 5
    workup.WAITafter 65 hr of fermentation

Vorschrift

The fermentation medium is a pineapple peel hydrolysate pretreated by dilute acid. For pretreatment, the concentration of the dilute acid is 2%, the operation temperature is 130° C., and the reaction time is 15 min at this temperature. In this case, NaOH is added to adjust the pH value of the hydrolysate to be 6.0, the fermentation temperature is controlled at 30° C., the agitation of the incubator is maintained at 100 rpm, and the ratio of the inocula size of the culture and the volume of the fermentation medium is 1:6 (v/v). The content of xylose in the pineapple peel hydrolysate is 19.8 g/L, and after 65 hr of fermentation, the amount of xylitol produced is 7.5 g/L, and the overall utilization of xylose is up to 97.4%, as shown in Table 4.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08409835B2uspto-grants-2013_04