Reaktion #2028933

ord-3dcf4fa8cc064b04bd70839642cf970b

Reaktionsgleichung

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
→
NCCCC[C@H](N)C(=O)O
L-Lysine

Edukte

CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
CCCCCCCC/C=C\CCCCCCCC(=O)[O-].[Na+]
sodium oleate
—
polyoxyethylene sorbitan monooleic acid ester
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](NC(=N)N)[C@@H](O)[C@@H]3NC(=N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O
streptomycin

Reagenzien

Keine

Lösungsmittel

OCC(O)CO
Glycerol

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigeequipped with baffles
  2. 2
    Sonstigeat 37° C.
  3. 3
    Sonstigefor 48 hours

Vorschrift

Glycerol stocks of the strains prepared in of Example 7 were thawed. 100 μL of each was evenly spread on an LB agar medium plate containing 40 mg/L ampicillin and 25 mg/L streptomycin and incubated at 37° C. for 24 hours. About ¼ volume of bacterial cells on the plate were inoculated into 40 mL of fermentation medium described below containing 40 mg/L ampicillin and 25 mg/L streptomycin in a 500 mL-Erlenmeyer flask equipped with baffles, and cultured using a reciprocal shaking culture apparatus at 37° C. for 48 hours. As a carbon source in a main culture, sodium oleate added with polyoxyethylene sorbitan monooleic acid ester (Tween 80: manufactured by NACALAI TESQUE, INC.) as an emulsification promoter to a final concentration of 0.5% (w/v) was used. As for a total amount of carbon sources, sodium oleate was 10 g/L. The medium composition used for the culture is shown below.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08389249B2uspto-grants-2013_03