Reaktion #2028927

ord-357a3c1794504bec86b7cf24772cf305

Reaktionsgleichung

C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C=O
L-Fucose
C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO
L-fucitol
Cl.NC(CO)(CO)CO
Tris-HCl
C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO
6-deoxy L-galactitol
→
CC(=O)[C@@H](O)[C@@H](O)[C@H](O)CO
1-deoxy D-tagatose

Edukte

C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C=O
L-Fucose
C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO
L-fucitol
Cl.NC(CO)(CO)CO
Tris-HCl
C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)CO
6-deoxy L-galactitol
—
221e

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

L-Fucose (6-deoxy L-galactose) was reduced, for a microbial oxidation of the resulting L-fucitol (6-deoxy L-galactitol) with E. agglomerans 221e [the bacterial cell at OD600=30, Tris-HCl buffer (pH 8.0) of 50 mM, the substrate 6-deoxy L-galactitol at 1%] at 30° C. for 6 hours. Only 1-deoxy D-tagatose was produced. FIG. 44(a) shows the reaction mixture after reaction, while FIG. 44 (b) shows the results of HPLC analysis of the produced 1-deoxy D-tagatose. The optical rotation was +14.0. 13C NMR spectrum thereof is shown in FIG. 45.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US08389248B2uspto-grants-2013_03