Reaktion #1995801

Reaktionsgleichung

5-Hydroxy-1-phenyl-1H-pyrazole-3-carboxylic acid

4-((S)-2-Amino-4-methanesulfonyl-butyryl)-piperazine-1-carboxylic acid ethyl ester

HOBT

DIPEA

→

4-{(S)-2-[(5-Hydroxy-1-phenyl-1H-pyrazole-3-carbonyl)-amino]-4-methanesulfonyl-butyryl}-piperazine-1-carboxylic acid ethyl ester

Lösungsmittel

DMF

EDC

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThen, the solvents were removed under reduced pressure
2. 2
   Sonstigethe crude product was purified by chromatography on silica gel eluting with a gradient of n-heptane/ethyl acetate
3. 3
   workup.ADDITIONThe fractions containing the product
4. 4
   Sonstigethe solvent evaporated under reduced pressure

Vorschrift

To a solution of 190 mg of 5-Hydroxy-1-phenyl-1H-pyrazole-3-carboxylic acid and 299 mg of 4-((S)-2-Amino-4-methanesulfonyl-butyryl)-piperazine-1-carboxylic acid ethyl ester in 3 ml of DMF, 142 mg of HOBT, 178 mg of EDC and 0.2 ml DIPEA was added and the reaction mixture was stirred for 16 h at RT. Then, the solvents were removed under reduced pressure and the crude product was purified by chromatography on silica gel eluting with a gradient of n-heptane/ethyl acetate. The fractions containing the product were combined and the solvent evaporated under reduced pressure. Yield: 327 mg.

Quelle