Reaktion #1918178

ord-982394f40b7d466ab77393233dc66ef7

Reaktionsgleichung

C/C(=C\c1csc(C)n1)[C@@H]1C[C@@H]2O[C@@H]2CCC[C@H](C)[C@H](O)[C@@H](C)C(=O)C(C)(C)[C@@H](O)CC(=O)O1
epothilone A
CO
methanol
→
C/C(=C\c1csc(CO)n1)[C@@H]1C[C@@H]2O[C@@H]2CCC[C@H](C)[C@H](O)[C@@H](C)C(=O)C(C)(C)[C@@H](O)CC(=O)O1
Epothilone E

Edukte

C/C(=C\c1csc(C)n1)[C@@H]1C[C@@H]2O[C@@H]2CCC[C@H](C)[C@H](O)[C@@H](C)C(=O)C(C)(C)[C@@H](O)CC(=O)O1
epothilone A
CO
methanol

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigeis 32° C.
  2. 2
    workup.ADDITIONAt the time of harvesting, 100 ml of XAD is added
  3. 3
    workup.WAITthe mixture is incubated further for 1 h
  4. 4
    SonstigeThe XAD is separated from the cells
  5. 5
    Waschenexhaustively eluted with methanol

Vorschrift

The concentrated culture is transferred to a 4 liter bioreactor and a methanolic solution of 1 g of epothilone A in 10 ml of methanol is added. The culture is then cultured further for a period of time of 21.5 h. The temperature is 32° C., the stirrer speed is 455 rpm and the introduction of air takes place at 6 l/min. At the time of harvesting, 100 ml of XAD is added and the mixture is incubated further for 1 h. The XAD is separated from the cells by screening and exhaustively eluted with methanol. The concentrated eluate is analysed by means of HPLC.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07067544B2uspto-grants-2006_06