Reaktion #1855696

ord-d5ecc4ec14794b97a139a22354e972dd

Reaktionsgleichung

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-biotin
O=P([O-])([O-])[O-]
phosphate
→
O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin

Edukte

O=C(CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21)NCCCCCC(=O)ON1C(=O)CCC1=O
NHS-LC-biotin
—
Example 5 ( a )
O=P([O-])([O-])[O-]
phosphate

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigethe mixture was reacted at 30° C. for 1 hour

Vorschrift

OA (manufactured by Sigma Co., 30 mg) was dissolved in 0.1M phosphate buffer (pH 7.0 , 1 ml), thereto was added an NHS-LC-biotin solution (manufactured by Pierce Chemical Co., 12.6 mg/ml 0.1M phosphate buffer, pH 7.0; 100 μl) and the mixture was reacted at 30° C. for 1 hour. Thereafter, the procedure in Example 5 (a) was repeated to give a biotin-OA fraction.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05378608uspto-grants-1995_01