Reaktion #1782276

Reaktionsgleichung

Amine

N-[4-(2-Amino-8-(3-aminopropyl)-9H-purin-6-yloxymethyl)-benzyl]-2,2,2-trifluoro-acetamide

N-(+)-biotinyl-6-aminocaproic acid succinimidyl ester

→

title compound

Ausbeute 86.0%

N-[4-(2-Amino-8-(3-(N-(+)-biotinyl-6-aminocaproyl)-aminopropyl)-9H-purine-6-yloxymethyl)-benzyl]-2,2,2-trifluoro-acetamide

Ausbeute 86.0%

Lösungsmittel

DMF

—

TEA

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.WAITleft at room temperature over night
2. 2
   SonstigeThe product is purified by reverse phase MPLC

Vorschrift

Amine 41 (Example 24, 10 mg, 0.023 mmol) and N-(+)-biotinyl-6-aminocaproic acid succinimidyl ester (50, 10 mg, 0.023 mmol) are dissolved in 650 μL DMF with 5 μL TEA and left at room temperature over night. The product is purified by reverse phase MPLC by using a linear gradient from water to acetonitrile (0.08% TFA) to yield 15.1 mg (0.019 mmol, 86%) of the title compound. MS (ESI) m/z 763.0 [M+H]+.

Quelle