Reaktion #1755446

Reaktionsgleichung

glyoxylic acid

L-cystine

borate

→

N,N,N′,N′-tetrakis(carboxymethyl)-L-cystine

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe alternative route, relying on in-solution phase synthesis of an intermediate product
2. 2
   Sonstigepurified with ion exchange chromatography

Vorschrift

The alternative route, relying on in-solution phase synthesis of an intermediate product starts with addition of a large excess (40 times) of glyoxylic acid to L-cystine in an alkaline borate buffer. The intermediate product, following pH manipulation and conductivity adjustment of the reaction mixture, purified with ion exchange chromatography to give N,N,N′,N′-tetrakis(carboxymethyl)-L-cystine.

Quelle