Reaktion #170650
ord-149252e9ce154b5e9ae76bde3686e059
Reaktionsgleichung
Reagenzien
Reaktionsbedingungen
Aufarbeitung
- 1SonstigeMass spectra were obtained on a ThermoFinnigan LCQ
Vorschrift
UV spectra were measured on a Perkin-Elmer Lambda 35 UV/VIS spectrometer. NMR experiments were conducted on a Bruker Avance AV300 NMR spectrometer operating at 300.13 MHz for 1H and 75.48 MHz for 13C using standard Bruker software. Mass spectra were obtained on a ThermoFinnigan LCQ utilizing both ESI and APCI in the positive and negative modes. HRESIMS was performed on a Micromass Q-TOF Ultima mass spectrometer. HPLC was done on a Waters 2695 using a Phenomenex Aqua column (250×4.6 mm, 5 μm) and monitored using a Waters 996 PDA scanning from 240 to 600 nm. Column chromatography was accomplished using Sephadex LH-20 (Pharmacia, 25-100 μm), reversed-phase C18 silica gel (J. T. Baker, 40 μm), and Diaion HP-20 (Mitsubishi, Japan). Separations were monitored using silica gel 60 F254 and RP18 F254 TLC plates (1 mm thickness, EM Science, Germany). Quercetin, rutin, cinnamic acid, O-coumaric acid, gallic acid, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma (MO, USA). Isoquercitrin and myricitrin were previously isolated in the laboratory (23).