Reaktion #164450
ord-ad6a8630d3f84495877d53ca549259a0
Reaktionsgleichung
Edukte
Reagenzien
Keine
Reaktionsbedingungen
Detaillierte Bedingungen
See reaction.notes.procedure_details.
Aufarbeitung
- 1workup.WAIT2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium
- 2WaschenLabeled cells were washed in serum-free culture medium and images
- 3Sonstigeequipped with a DP50 digital camera)
- 4Filtrationfilter
Vorschrift
2.5-5×105 cells were incubated over-night at 29° C. in 0.5 mL of culture medium containing 10 mM fluorescein-labeled methotrexate (F-MTX, Molecular Probes/Invitrogen). Labeled cells were washed in serum-free culture medium and images were recorded using a fluorescence microscope (Olympus CKX41 microscope equipped with a DP50 digital camera) using a FITC filter set.