Reaktion #1534599

Reaktionsgleichung

benzoyl-L-isoleucine

L-proline

dicyclohexylamine

proline

dicyclohexyl-carbodiimide

isoleucine

allo-isoleucine

→

Benzoyl-D-allo-Isoleucyl-L-proline dicyclohexyl-ammonium salt

Lösungsmittel

methylene chloride

triethyl amine

triethylamine

—

petroleum ether

Reaktionsbedingungen

Aufarbeitung

1. 1
   FiltrationThe precipitated dicyclohexyl-urea is filtered off
2. 2
   Waschenwashed with petroleum ether (2×20 ml.)
3. 3
   ExtraktionThe filtrate is extracted with water (2×50 ml.), 5 percent sodium hydrogen carbonate solution (2×50 ml.) and water
4. 4
   Trocknendried over sodium sulfate
5. 5
   Sonstigeevaporated at reduced pressure
6. 6
   workup.DISSOLUTIONThe residue is dissolved in 80 ml
7. 7
   workup.STIRRINGThe reaction mixture is stirred at room temperature for six hours
8. 8
   Sonstigeis evaporated under reduced pressure
9. 9
   workup.DISSOLUTIONThe residue is dissolved in a mixture of water (100 ml.) and ether (50 ml.)
10. 10
    Waschenthe aqueous phase is washed twice with ether (20 ml.)
11. 11
    Waschenthe combined ether extracts are washed twice with water (20 ml.)
12. 12
    ExtraktionThe separated oil is extracted three times with ethyl acetate (50 ml.)
13. 13
    Waschenthe combined ethyl acetate is washed twice with water (20 ml.)
14. 14
    Trocknendried over sodium sulfate
15. 15
    Sonstigeevaporated under reduced pressure
16. 16
    workup.DISSOLUTIONThe residue is dissolved in 100 ml
17. 17
    workup.ADDITIONof dicyclohexyl amine are poured to the solution
18. 18
    SonstigeThe crystals formed
19. 19
    Filtrationare filtered
20. 20
    Waschenwashed with ether (3×20 ml.)
21. 21
    Trocknendried over concentrated sulfuric acid in vacuo
22. 22
    Sonstige(64 percent of the theoretical yield) of the named compound

Vorschrift

19.2 g. (81.5 mmoles) of benzoyl-L-isoleucine (F. Ehrlich: Berichte 37, 1809 (1904) and 16.5 g. (80 mmoles) of dicyclohexyl-carbodiimide are dissolved in 150 ml. of methylene chloride cooled to 5° to 10° C. The solution is stirred in an ice bath for two hours, then 2 ml. of triethyl amine and 100 ml. of petroleum ether are added. The precipitated dicyclohexyl-urea is filtered off and washed with petroleum ether (2×20 ml.). The filtrate is extracted with water (2×50 ml.), 5 percent sodium hydrogen carbonate solution (2×50 ml.) and water, dried over sodium sulfate and evaporated at reduced pressure. The residue is dissolved in 80 ml. of pyridine, then 9.2 g. (80 mmoles) of L-proline and 22.4 ml. (160 mmoles) of triethylamine are added to the solution. The reaction mixture is stirred at room temperature for six hours, then is evaporated under reduced pressure. The residue is dissolved in a mixture of water (100 ml.) and ether (50 ml.), the aqueous phase is washed twice with ether (20 ml.), and the combined ether extracts are washed twice with water (20 ml.). The aqueous phases are combined and acidified with 5 N sulfuric acid to pH=2. The separated oil is extracted three times with ethyl acetate (50 ml.), the combined ethyl acetate is washed twice with water (20 ml.), dried over sodium sulfate and evaporated under reduced pressure. The residue is dissolved in 100 ml. of ether, thereafter 16 ml. of dicyclohexyl amine are poured to the solution. The crystals formed are filtered, washed with ether (3×20 ml.) and dried over concentrated sulfuric acid in vacuo. Yield 26.3 g. (64 percent of the theoretical yield) of the named compound. M.p. 117° to 118° C. RF1 =0.36 to 0.46 and 0.13 to 0.23 (dicyclohexylamine). Amino acid analysis: proline: 1.00 (base); allo-isoleucine: 0.92; isoleucine: 0.02.

Quelle