Reaktion #1528

ord-b36cf4c3fce444e59afcc58bc7669a0f

Reaktionsgleichung

CCO
ethanol
CCO
ethanol
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
→
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

Edukte

CCO
ethanol
CCO
ethanol
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose
O=C[C@H](O)[C@@H](O)[C@H](O)CO
xylose

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.ADDITIONcontaining the shuttle vector alone (CP4[pZB 186]), the recombinant

Vorschrift

The results presented in FIG. 2 show that in contrast to the control strain containing the shuttle vector alone (CP4[pZB 186]), the recombinant containing the added xylose isomerase, xylulokinase, transaldolase and transketolase genes demonstrated growth and ethanol production from xylose as a carbon source. The recombinant strain produces ethanol from glucose as efficiently as the control strain at 94% of theoretical yield. The recombinant strain additionally produces ethanol from xylose at 84% of theoretical yield in 79 hours. Furthermore, in the combined presence of glucose and xylose, the recombinant strain ferments both sugars simultaneously to ethanol at 88% of theoretical yield within 48 hours, thus providing the foundation for advanced process designs with cofermentation of mixed-sugar feedstocks.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05726053uspto-grants-1998_03