Reaktion #1527485

ord-b513f79847d247d484ea46dfd5b6252f

Reaktionsgleichung

CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O
spectinomycin
O=P([O-])([O-])O.[K+].[K+]
K2HPO4
O=P([O-])(O)O.[K+]
KH2PO4
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
O=C([O-])[O-].[Ca+2]
CaCO3
OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O
lactose
→
NCCC[C@H](N)C(=O)O
L-ornithine

Edukte

CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O
spectinomycin
O=P([O-])([O-])O.[K+].[K+]
K2HPO4
—
(NH4)2SO4
O=P([O-])(O)O.[K+]
KH2PO4
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
O=C([O-])[O-].[Ca+2]
CaCO3
OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O
lactose

Reagenzien

Keine

Reaktionsbedingungen

Temperatur
30°CELSIUS
Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    workup.STIRRINGshaking culture
  2. 2
    workup.WAITwas carried out at 30° C. for 72 hours

Vorschrift

Each strain was cultured with shaking in NB medium at 30° C. for 16 hours. For the culture of the transformant, spectinomycin was added to NB medium at a concentration of 100 μg/ml. Then, 0.5 ml of the seed culture was inoculated into 5 ml of a production medium (pH 7.0) comprising 80 g/l lactose, 40 g/l (NH4)2SO4, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 100 mg/l L-arginine and 20 g/l CaCO3 in a test tube, and shaking culture was carried out at 30° C. for 72 hours. After the culturing was finished, the culture filtrate was subjected to paper chromatography and the amount of L-ornithine formed was determined colorimetrically by using the color development with ninhydrin. The results are shown in Table 20.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05498532uspto-grants-1996_03