Reaktion #1503043

ord-2e10469a48be465f8c33b26268df6fbf

Reaktionsgleichung

N[C@@H](CCC(=O)O)C(=O)O
Glutamic acid
CC(=O)c1ccc[n+](C2OC(COP(=O)(O)OP(=O)(O)OCC3OC(n4cnc5c(N)ncnc54)C(O)C3O)C(O)C2O)c1
3-acetylpyridine NAD
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
CC(=O)c1cccnc1.NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
3-acetylpyridine NADH

Edukte

N[C@@H](CCC(=O)O)C(=O)O
Glutamic acid
CC(=O)c1ccc[n+](C2OC(COP(=O)(O)OP(=O)(O)OCC3OC(n4cnc5c(N)ncnc54)C(O)C3O)C(O)C2O)c1
3-acetylpyridine NAD
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

Glutamic acid was determined in the same manner as in Example 3 except that enzyme reaction was carried at 50° C. for 20 minutes in the presence of 3-acetylpyridine NAD (produced by Sigma Co. Ltd.) as coenzyme for glutamate, dehydrogenase (i.e., 3-acetylpyridine NADH formed by enzyme reaction was used as an indicator). The results had a good linear relationship to the real concentrations of glutamic acid. The measurements could be effected in a very short time (about 30 seconds).

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US05909114uspto-grants-1999_06