Reaktion #1500373

ord-a946dab995e7443c91c9aafda8641185

Reaktionsgleichung

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
adenosine triphosphate
C=C(OP(=O)(O)O)C(=O)O
phosphoenol pyruvate
CC(=O)C(=O)[O-]
pyruvate
CC(=O)OCC1=C(C(=O)O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(=O)O)[C@H]2SC1
cephalosporin C
CC(=O)OCC1=C(C(=O)O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(=O)O)[C@H]2SC1
cephalosporin C
→
CC1(C)S[C@@H]2[C@H](NC(=O)CCC[C@H](N)C(=O)O)C(=O)N2[C@H]1C(=O)O
Isopenicillin N

Edukte

—
β-lactam
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
adenosine triphosphate
C=C(OP(=O)(O)O)C(=O)O
phosphoenol pyruvate
CC(=O)C(=O)[O-]
pyruvate
CC(=O)OCC1=C(C(=O)O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(=O)O)[C@H]2SC1
cephalosporin C
CC(=O)OCC1=C(C(=O)O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(=O)O)[C@H]2SC1
cephalosporin C

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Extraktionextract
  2. 2
    Sonstigeproduced in accordance with the procedure
  3. 3
    Sonstigeproduces the same size inhibitory zone

Vorschrift

Five μmoles of adenosine triphosphate (ATP), 10 μmoles of phosphoenol pyruvate, and 100 μgrams of pyruvate kinase were incubated for three hours at 25° C. at a pH of 7.2 with 100 μg of "LLD" and 1.0 ml of mutant M-0198 extract produced in accordance with the procedure set forth above. The reaction mixture showed the presence of antibiotic activity equivalent to 2.3 μg/ml using the β-lactam antibiotic, cephalosporin C, as standard i.e. 2.3 units/ml. One unit of antibiotic activity is that which produces the same size inhibitory zone as does 1 μg of cephalosporin C.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US04248966uspto-grants-1981_02