Reaktion #1491253

Reaktionsgleichung

streptomycin

O2

L-glutamine

EDTA

penicillin

→

Albendazole

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeSKOV-3 human cystadenocarcinoma cell line, obtained from the American Type Culture Collection (ATCC Accession No. HTB 77)
2. 2
   SonstigeTwenty-four hours later, the medium was removed
3. 3
   WaschenSubconfluent cultures were washed three times with phosphate buffer
4. 4
   workup.ADDITIONcontaining various
5. 5
   Einengenconcentrations of albendazole (0, 0.1, 0.25 and 1.0 μM)
6. 6
   workup.DISSOLUTIONdissolved in 1% ethanol
7. 7
   SonstigeAfter completion of the treatment period, medium from the wells were individually collected
8. 8
   Einengenanalysed for the VEGF concentration

Vorschrift

SKOV-3 human cystadenocarcinoma cell line, obtained from the American Type Culture Collection (ATCC Accession No. HTB 77) were grown in McCoy's 5a medium with 1.5 mM L-glutamine, 100 units/ml penicillin, and 100 μg/ml streptomycin, supplemented with 10% FCS. Cells were grown to confluence and harvested by trypsinization with 0.25 mg/ml trypsin/EDTA and suspended in the medium before plating. These were then seeded (2×105) on plastic 6-well Corning culture plates. Cultures were maintained in a 37° C. incubator in a humidified atmosphere of 95% O2/5% CO2. Twenty-four hours later, the medium was removed. Subconfluent cultures were washed three times with phosphate buffer followed by incubation for 6 hours with culture medium containing various concentrations of albendazole (0, 0.1, 0.25 and 1.0 μM) dissolved in 1% ethanol. After completion of the treatment period, medium from the wells were individually collected and analysed for the VEGF concentration using an enzyme-linked immunosorbant assay (ELISA) that detects soluble VEGF121 and VEGF165 isoforms (Quantikine R&D systems, Minneapolis, USA).

Quelle