Reaktion #1382736

Reaktionsgleichung

title compound

7-(2′-methyl-β-D-ribofuranosyl)-4-amino-5-(trimethylsilanylethyn-1-yl)-pyrrolo[2,3-d]pyrimidine

NH4OH

→

7-(2′-C-methyl-β-D-ribofuranosyl)-4-amino-5-(ethyn-1-yl)-pyrrolo[2,3-d]pyrimidine

Lösungsmittel

methanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe solvent was evaporated
2. 2
   workup.DISSOLUTIONthe residue dissolved in methanol and co-evaporated with silica gel
3. 3
   Filtrationfilter with silica gel
4. 4
   Waschenthe desilylated compound was eluted with acetone
5. 5
   SonstigeSolvent was evaporated
6. 6
   Sonstigethe residue crystallized from methanol/acetonitrile

Vorschrift

The title compound from Example 2 (0.040 g, 0.0132 mmol) was dissolved in methanol and NH4OH was added and the mixture left at ambient temperature for 1 hour. The solvent was evaporated, the residue dissolved in methanol and co-evaporated with silica gel. Dry silica was loaded on the glass filter with silica gel and the desilylated compound was eluted with acetone. Solvent was evaporated and the residue crystallized from methanol/acetonitrile to provide for 7-(2′-C-methyl-β-D-ribofuranosyl)-4-amino-5-(ethyn-1-yl)-pyrrolo[2,3-d]pyrimidine.

Quelle