Reaktion #11947

Reaktionsgleichung

WSCI hydrochloride

compound

5-(N-Boc-N-2-picolylaminomethyl)pyrazine-2-carboxylic acid

diethylamine

compound

Nδ-Cbz-L-ornithine

→

title compound

Nα-(5-(N-Boc-N-2-picolylaminomethyl)pyrazine-2-carbonyl)-Nδ-Cbz-L-ornithine

Lösungsmittel

DMF

DMF

DMF

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigethe residue obtained
2. 2
   workup.WAITAfter 15 hours
3. 3
   Einengenthe reaction solution was concentrated
4. 4
   workup.ADDITIONAfter the addition of chloroform and 1 mol/l hydrochloric acid
5. 5
   Extraktionthe residue was extracted with chloroform
6. 6
   WaschenThe organic layer was washed with saturated brine
7. 7
   Trocknendried over anhydrous sodium sulfate
8. 8
   SonstigeThe residue was crude-purified by silica gel column chromatography (4 g, chloroform/ethyl acetate=1/2)

Vorschrift

The compound obtained in Example 8-6 (160 mg) was dissolved in DMF (3.2 ml) and diethylamine (0.32 ml) was added. After one hour, the reaction solution was concebtrated and the residue obtained was dissolved in DMF (1 ml). Then, WSCI hydrochloride (73 mg), DMAP (31 mg), and a solution of the compound obtained in Example 13-3 in DMF (1 ml) were sequentially added. After 15 hours, the reaction solution was concentrated. After the addition of chloroform and 1 mol/l hydrochloric acid, the residue was extracted with chloroform. The organic layer was washed with saturated brine and dried over anhydrous sodium sulfate. The residue was crude-purified by silica gel column chromatography (4 g, chloroform/ethyl acetate=1/2) to obtain the title compound (95.2 mg) as colorless syrup.

Quelle