Reaktion #1154364

Reaktionsgleichung

bromoacetic acid

trimethoxy-(3-aminopropyl)silane

→

trimethoxy-3-bromoacetamido-propylsilane

Lösungsmittel

1-(3-dimethylaminopropyl)-3-ethylcarbodiimide-hydrochloride

methylene chloride

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeThe linker product was purified by column chromatography on silica gel
2. 2
   WaschenThe resulting linker-bound substrate was thoroughly rinsed with pure toluene and methylene chloride
3. 3
   Sonstigedried for about 18 hours under reduced pressure

Vorschrift

Binder DNA oligonucleotide-derivatized porous silicon films were employed to test the selectivity and limits of detection of a contemplated sensor. For attachment of DNA, a trimethoxy-3-bromoacetamido-propylsilane linker was synthesized by reaction of bromoacetic acid with trimethoxy-(3-aminopropyl)silane in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide-hydrochloride in methylene chloride as solvent. The linker product was purified by column chromatography on silica gel. The oxidized porous silicon samples were then contacted with a toluene solution of the linker for 2 hours. The resulting linker-bound substrate was thoroughly rinsed with pure toluene and methylene chloride, and dried for about 18 hours under reduced pressure.

Quelle