Reaktion #11403

Reaktionsgleichung

isoamyl nitrite

potassium tert-butoxide

potassium

oestrone

→

16-Oximino-Estrone

Lösungsmittel

water

tert-butanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   workup.ADDITIONwas added
2. 2
   SonstigeThe deep red mixture obtained
3. 3
   workup.STIRRINGwas stirred overnight
4. 4
   ExtraktionThe resulting solution was extracted with ether (2×20 mL)
5. 5
   Sonstige(10 mL) to give a light yellow precipitate
6. 6
   workup.WAITThis was left
7. 7
   Sonstigeseparating for two hours after which the solid
8. 8
   Filtrationwas filtered (140 mg, 63%)
9. 9
   Sonstigemp 223–225° C. (lit. 226–227° C.);43 TLC (chloroform/acetone, 9:1) Rf 0.27

Vorschrift

To a stirred solution of potassium tert-butoxide under an atmosphere of N2, freshly prepared by dissolving potassium metal (80 mg, 2.05 mmol) in 2 mL tert-butanol, oestrone (200 mg, 740 mmol) was added. The reaction mixture was then stirred for 1 hour at room temperature under N2 and isoamyl nitrite (180 μmol, 1.34 mmol) was added dropwise. The deep red mixture obtained was stirred overnight and then poured into water (20 mL). The resulting solution was extracted with ether (2×20 mL) and the aqueous layer was acidified with glacial acetic acid. (10 mL) to give a light yellow precipitate. This was left separating for two hours after which the solid was filtered (140 mg, 63%): mp 223–225° C. (lit. 226–227° C.);43 TLC (chloroform/acetone, 9:1) Rf 0.27 cf. Rf 0.69 (E1); IR (KBr) 3385 (NOH), 2920–2860 (aliph CH), 1735 (C═O), 1605–1500 (arom C═C) cm−1; δH (DMSO-d6, 400 MHz) 0.89 (3H, s, C-18-H3), 1.30–2.85 (11H, m), 2.70–2.81 (2H, m, C-6-H2), 6.46 (1H, d, JC-2-H,C-4-H=2.3 Hz, C-4-H), 6.52 (1H, dd, JC-1-H,C-2-H=8.4 Hz and JC-4-H,C-2-H=2.3 Hz, C-2-H), 7.05 (1H, d, JC-2-H,C-1-H=8.2 Hz, C-1-H), 9.05 (1H, s, exchanged with D2O, OH) and 12.39 (1H, s, exchanged with D2O, NOH); δc (DMSO-d6, 100.4 MHz) 14.09 (q, C-18), 25.09 (t); 25.46 (t), 26.18 (t), 29.02 (t), 30.92 (t), 37.20 (d), 43.20 (d), 44.59 (d), 48.50 (s, C-13), 112.70 (d), 114.83 (d), 125.82 (d), 129.59 (s), 136.88. (s), 154.84 (s, C-3 or C-16), 155.23 (s, C-3 or C-16) and 204.64 (s, C═O); MS m/z (FAB+) 453.2 [30, (M+H+NBA)+], 300.1 [100, (M+H)+]; MS m/z (FAB−) 451.3 [38, (M−H+NBA)−], 298.2 [100, (M−H)−]; Acc MS m/z (FAB+) 300.15963, C18H22NO3 requires 300.115997. CHN,

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