Reaktion #1129470

Reaktionsgleichung

4-(8-(3-(1-(4-methoxybenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-yl)pyridin-2-ylamino)-7-methylquinazolin-4-ylamino)benzonitrile

→

4-(8-(3-(1H-pyrazolo[3,4-d]pyrimidin-4-yl)pyridin-2-ylamino)-7-methylquinazolin-4-ylamino)benzonitrile

Lösungsmittel

TFA

Reaktionsbedingungen

Aufarbeitung

1. 1
   SonstigeTFA was removed under vacuum
2. 2
   workup.ADDITIONMeOH (2N, NH3) (20 ml) was added
3. 3
   Sonstigesolvent was removed under vacuum
4. 4
   SonstigeThe product was purified by flash chromatography
5. 5
   Wascheneluting with MeOH/DCM (1-5%)

Vorschrift

A mixture of 4-(8-(3-(1-(4-methoxybenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-yl)pyridin-2-ylamino)-7-methylquinazolin-4-ylamino)benzonitrile (120 mg, 203 μmol) in TFA (3 ml) was heated at 65° C. for 3 hr. TFA was removed under vacuum. MeOH (2N, NH3) (20 ml) was added and then solvent was removed under vacuum. The product was purified by flash chromatography eluting with MeOH/DCM (1-5%) to give 4-(8-(3-(1H-pyrazolo[3,4-d]pyrimidin-4-yl)pyridin-2-ylamino)-7-methylquinazolin-4-ylamino)benzonitrile. MS (ESI pos. ion) m/z 471 (M+H+).

Quelle