Reaktion #1129455

Reaktionsgleichung

HCl

4-(5-amino-6-methylisoquinolin-1-ylamino)benzonitrile

9-(4-methoxybenzyl)-6-(2-fluoropyridin-3-yl)-9H-purine

NaHCO3

lithium bis(trimethylsilyl)amide

→

4-(5-(3-(9-(4-methoxybenzyl)-9H-purin-6-yl)pyridin-2-ylamino)-6-methylisoquinolin-1-ylamino)benzonitrile

Lösungsmittel

THF

Reaktionsbedingungen

Aufarbeitung

1. 1
   Sonstigeto give a dark suspension
2. 2
   SonstigeThe suspension was sonicated for 2 min
3. 3
   SonstigeThe reaction mixture was sonicated again for 2 min
4. 4
   workup.STIRRINGstirred at RT for 10 min
5. 5
   Extraktionextracted with 3:1 CHCl3
6. 6
   WaschenThe combined organic layers were washed with satd NaCl
7. 7
   Trocknendried over Na2SO4
8. 8
   Filtrationfiltered
9. 9
   Einengenconcentrated in vacuo
10. 10
    Sonstigeto give 840 mg crude product
11. 11
    Sonstigechromatographed through a Redi-Sep® pre-packed silica gel column (80 g)
12. 12
    Wascheneluting with a gradient of 10% to 50% EtOAc in CH2Cl2

Vorschrift

A mixture of 4-(5-amino-6-methylisoquinolin-1-ylamino)benzonitrile (0.250 g, 0.91 mmol) and 9-(4-methoxybenzyl)-6-(2-fluoropyridin-3-yl)-9H-purine (0.34 g, 1.0 mmol) in anhydrous THF (20 mL) was treated dropwise with lithium bis(trimethylsilyl)amide (1.0 M solution in THF; 2.7 mlL, 2.7 mmol) to give a dark suspension. The suspension was sonicated for 2 min and then stirred at RT for 30 min. The reaction mixture was sonicated again for 2 min. The mixture was treated with 1N aq HCl (5 mL) and stirred at RT for 10 min. The reaction mixture was added to satd NaHCO3 (70 mL) and extracted with 3:1 CHCl3:IPA (3×50 mL). The combined organic layers were washed with satd NaCl, dried over Na2SO4, filtered, and concentrated in vacuo to give 840 mg crude product. The crude product was adsorbed onto a plug of silica gel and chromatographed through a Redi-Sep® pre-packed silica gel column (80 g), eluting with a gradient of 10% to 50% EtOAc in CH2Cl2, to give 4-(5-(3-(9-(4-methoxybenzyl)-9H-purin-6-yl)pyridin-2-ylamino)-6-methylisoquinolin-1-ylamino)benzonitrile. MS (ESI, pos. ion) m/z: 590.2 [M+H]+

Quelle