Reaktion #1076751

ord-e6fdf8cb52054fadae9dd84aa0bbce9b

Reaktionsgleichung

NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
→
CC(C)=CCC/C(C)=C/CC/C(C)=C/CC/C=C(\C)CC/C=C(\C)CCC=C(C)C
MF59

Edukte

—
MF59-0
NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
—
2
—
MF59-0

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigeto yield a total of 0.7 ml
  2. 2
    workup.ADDITIONThe composition was gently mixed
  3. 3
    Sonstigeproduced in Example 1
  4. 4
    Sonstigeto yield a total of 3 ml
  5. 5
    workup.ADDITIONThe composition was gently mixed

Vorschrift

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2×phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US06458370B1uspto-grants-2002_10