Reaktion #10462

ord-0e53689819a44ea5b6020ddf049fe8f2

Reaktionsgleichung

NC(=O)CC[C@H](N)C(=O)O
glutamine
CCN
ethylamine
→
CCNC(=O)CC[C@H](N)C(=O)O
L-theanine

Edukte

NC(=O)CC[C@H](N)C(=O)O
glutamine
CCN
ethylamine

Reagenzien

Keine

Lösungsmittel

[O-]B([O-])[O-]
borate

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

0.3 M glutamine and 1.5 M ethylamine were allowed to react at 30° C. for 22 hours in borate buffer (Na2B4O7—NaOH, pH 11) in the presence of 0.3 U glutaminase, to give 225 nmol of L-theanine. In addition, a by-product glutamic acid was 20 nmol. The purification of theanine from the reaction mixture was carried out by subjecting the reaction mixture to column chromatography using Dowex 50×8 column and Dowex 1×2 column, and thereafter treating the resulting product with ethanol.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07094787B2uspto-grants-2006_08