Reaktion #10257

ord-2c4f74d36a3e4147b27538559e142388

Reaktionsgleichung

[Cl-].[Na+]
NaCl
O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)O
LTA4
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)OC
LTA4 methyl ester
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)O
LTA4
→
CCCCC/C=C\C[C@@H](O)/C=C/C=C/C=C\[C@@H](O)CCCC(=O)O
LTB4

Edukte

—
NaN2
[Cl-].[Na+]
NaCl
O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
EDTA
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)O
LTA4
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)OC
LTA4 methyl ester
CCCCC/C=C\C\C=C/C=C/C=C/[C@@H]1O[C@H]1CCCC(=O)O
LTA4

Reagenzien

Keine

Lösungsmittel

O=P([O-])([O-])[O-].[K+].[K+].[K+]
potassium phosphate

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Vorschrift

The LTA4 substrate was freshly prepared by alkaline hydrolysis of LTA4 methyl ester (Cayman Chemical Co., USA). After incubating for 10 minutes in the presence of LTA4 (1 μM in 50 mM Tris-HCl, 0.15 M NaCl, 0.5% BSA, pH 7.4), the reaction is stopped by diluting 1/20 in 0.1 M potassium phosphate buffer containing 1.5 mM NaN2, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA, pH 7.4, −4° C.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US07094773B2uspto-grants-2006_08