Reaktion #1013868

ord-3844cc9d50d1424fa227ef18187debb2

Reaktionsgleichung

COc1cccc2c1C(=O)c1c(O)c3c(c(O)c1C2=O)C[C@@](O)(C(=O)CO)C[C@@H]3O[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1
doxorubicin
O=C(O)Cc1cc(I)c(Oc2cc(I)c(O)c(I)c2)c(I)c1
tetrac
C=O
formalin
→
OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O
β-Gal

Edukte

COc1cccc2c1C(=O)c1c(O)c3c(c(O)c1C2=O)C[C@@](O)(C(=O)CO)C[C@@H]3O[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1
doxorubicin
O=C(O)Cc1cc(I)c(Oc2cc(I)c(O)c(I)c2)c(I)c1
tetrac
C=O
formalin

Reagenzien

Keine

Reaktionsbedingungen

Detaillierte Bedingungen
See reaction.notes.procedure_details.

Aufarbeitung

  1. 1
    Sonstigeat 37° C.
  2. 2
    Sonstigefor 3 min
  3. 3
    Waschenwashed with PBS
  4. 4
    workup.ADDITIONtheir fractions between non treated
  5. 5
    workup.ADDITIONtreated populations

Vorschrift

Cells were seeded on 100-mm culture dishes and treated with doxorubicin, tetrac or both for 24 h, after which the cells were subjected to trypsin treatment at 37° C. for 3 min, fixed with 4% formalin in PBS, and washed with PBS. Then they were incubated with 0.1 μg/ml Hoechst 33248 (bisbenzimide, Sigma-Aldrich) and spotted on slides for microscopy. Positive cells are counted and their fractions between non treated and treated populations are compared.

Quelle

DOI: 10.6084/m9.figshare.5104873.v1Patent: US09289395B2uspto-grants-2016_03