Reaktion #1009592

Reaktionsgleichung

2-(3-chloro-5-(piperazin-1-yl)phenyl)-5,7-dimethoxyquinazolin-4(3H)-one

2-(3-Chloro-5-(piperazin-1-yl)phenyl)-5,7-dimethoxyquinazolin-4(3H)-one

acetone

sodium acetate

acetic acid

sodium triacetoxyborohydride

→

title compound

Ausbeute 18.0%

2-(3-Chloro-5-(4-isopropylpiperazin-1-yl)phenyl)-5,7-dimethoxyquinazolin-4(3H)-one

Ausbeute 18.0%

Lösungsmittel

1,2-dichloroethane methanol

Reaktionsbedingungen

Aufarbeitung

1. 1
   Einengenconcentrated
2. 2
   SonstigeThe residue was purified by silica gel chromatography
3. 3
   Wascheneluting with 0-100% 92:7:1 CHCl3/MeOH/concentrated NH4OH in CH2Cl2
4. 4
   SonstigeThis material was further purified by reverse phase HPLC
5. 5
   Wascheneluting with 10% to 90% CH3CN in H2O with 0.1% TFA
6. 6
   EinengenThe product fractions were concentrated
7. 7
   workup.DISSOLUTIONthe residue was dissolved in acetonitrile
8. 8
   Einengenconcentrated
9. 9
   SonstigeThe residue was purified by reverse phase HPLC
10. 10
    Wascheneluting with 10% to 90% CH3CN in H2O without TFA

Vorschrift

To a solution of 2-(3-chloro-5-(piperazin-1-yl)phenyl)-5,7-dimethoxyquinazolin-4(3H)-one (Example 31, 0.054 g, 0.14 mmol), acetone (0.060 g, 1.4 mmol), sodium acetate (0.023 g, 0.27 mmol), and acetic acid (0.16 g, 2.7 mmol) in 1,2-dichloroethane/methanol (5/5 mL) was added sodium triacetoxyborohydride (0.56 g, 2.7 mmol). The mixture was stirred at room temperature for 16 h and then concentrated. The residue was purified by silica gel chromatography eluting with 0-100% 92:7:1 CHCl3/MeOH/concentrated NH4OH in CH2Cl2. This material was further purified by reverse phase HPLC eluting with 10% to 90% CH3CN in H2O with 0.1% TFA. The product fractions were concentrated and the residue was dissolved in acetonitrile and water. The mixture was basified with concentrated NH4OH and concentrated. The residue was purified by reverse phase HPLC eluting with 10% to 90% CH3CN in H2O without TFA to afford the title compound (0.011 g, 18%) as an off-white solid: 1H NMR (500 MHz, CD3OD) δ 7.52 (s, 1H), 7.49 (d, J=1.5 Hz, 1H), 7.18 (t, J=1.5 Hz, 1H), 6.82 (d, J=2.5 Hz, 1H), 6.59 (d, J=2.5 Hz, 1H), 3.93 (s, 3H), 3.92 (s, 3H), 3.35-3.60 (br s, 4H), 2.85-3.15 (br s, 4H), 1.25-1.40 (br s, 1H), 1.23 (d, J=6.5 Hz, 6H); ESI MS m/z 443 [M+H]+.

Quelle