تفاعل #948994

معادلة التفاعل

1,1,1-tris(hydroxymethyl)ethane

potassium phosphate

1,1,1-tris(hydroxymethyl)ethane

→

2,2-bis (hydroxymethyl)propionic acid

2,2-bis (hydroxymethyl)propionaldehyde

ظروف التفاعل

المعالجة

1. 1
   workup.ADDITIONwas added
2. 2
   أخرىto prepare
3. 3
   أخرىa resting cell suspension reaction mixture
4. 4
   workup.STIRRINGThis was shaken at 30° C
5. 5
   أخرىhad been oxidized at 66 hours of the reaction

الإجراء التجريبي

One loopful bacterial cells of Agrobacterium sp. SD807 strain were inoculated in 5 ml of a culture medium having the same composition as culture medium 2 shown in Table 3 and incubated with shaking at 30° C. for 1 day. After completion of the cultivation, the bacterial cells were harvested by centrifugation and a 50 mM potassium phosphate buffer solution containing 1,1,1-tris(hydroxymethyl)ethane in a concentration of 5 g/l was added thereto to prepare a resting cell suspension reaction mixture. This was shaken at 30° C. The reaction mixture had a turbidity in terms of absorbance of 0.2 at 660 nm. A portion of the reaction mixture was analyzed by high performance liquid chromatography and as a result, it was found that 4.9 g/l of 1,1,1-tris(hydroxymethyl)ethane had been oxidized at 66 hours of the reaction to produce 0.1 g/l of 2,2-bis (hydroxymethyl)propionaldehyde and 4.8 g/l of 2,2-bis (hydroxymethyl)propionic acid. Conversion rate of 1,1,1-tris(hydroxymethyl)ethane to 2,2-bis(hydroxymethyl)propionic acid was about 88%.

المصدر