تفاعل #948993

معادلة التفاعل

1,1,1-tris(hydroxymethyl)ethane

potassium phosphate

1,1,1-tris(hydroxymethyl)ethane

→

2,2-bis (hydroxymethyl)propionaldehyde

2,2-bis (hydroxymethyl)propionic acid

ظروف التفاعل

المعالجة

1. 1
   workup.ADDITIONwas added
2. 2
   أخرىto prepare
3. 3
   أخرىa resting cell suspension reaction mixture
4. 4
   workup.STIRRINGThis was shaken at 30° C. for 24 hours

الإجراء التجريبي

One loopful bacterial cells of Rhodococcus erythropolis SD806 strain were inoculated in 200 ml of a culture medium having the same composition as culture medium 1 shown in Table 3 and incubated with shaking at 30° C. for 2 days. After completion of the incubation, the bacterial cells were harvested by centrifugation and 1 liter of a 50 mM potassium phosphate buffer solution containing 5 g of 1,1,1-tris(hydroxymethyl)ethane was added thereto to prepare a resting cell suspension reaction mixture. The reaction mixture had a turbidity in terms of absorbance of 0.8 at 660 nm. This was shaken at 30° C. for 24 hours. A portion of the reaction mixture was analyzed by high performance liquid chromatography and as a result, it was found that 3.3 g of 1,1,1-tris(hydroxymethyl)ethane was oxidized and decomposed to produce 1.1 g of 2,2-bis (hydroxymethyl)propionaldehyde and 2.0 g of 2,2-bis (hydroxymethyl)propionic acid.

المصدر