تفاعل #94670

معادلة التفاعل

NAD

phthalimide

hydrochloric acid

→

4-(3-aminopropyl)-pyrazole dihydrochloride

ظروف التفاعل

المعالجة

1. 1
   أخرىphthalic acid removed by filtration
2. 2
   أخرىthe filtrate evaporated to dryness
3. 3
   أخرىto give a product which
4. 4
   أخرىafter recrystallization
5. 5
   workup.ADDITIONfrom a mixture of ethanol and ethyl ether
6. 6
   أخرىThis pyrazole compound could be coupled to the activated dextran by reaction of the terminal amino group

الإجراء التجريبي

This phthalimide intermediate (8.0 gram, 0.03 mole) was refluxed in 100 ml. of 6 normal hydrochloric acid overnight. The clear solution was cooled to 4° C., phthalic acid removed by filtration, and the filtrate evaporated to dryness to give a product which, after recrystallization from a mixture of ethanol and ethyl ether, amounted to 4.4 grams of a white solid identified as 4-(3-aminopropyl)-pyrazole dihydrochloride, melting point 189°-190° C. This pyrazole compound could be coupled to the activated dextran by reaction of the terminal amino group, but the resulting affinity resin, in which the coupling arm had a length of only 6 Angstrom units, had poor binding capacity for the binary complex of alcohol dehydrogenase and NAD.

المصدر