تفاعل #930684

ord-54b0018727104f60bb435e501f24b507

معادلة التفاعل

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP
→
Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ATP

المتفاعلات

Nc1ncnc2c1ncn2[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]1O
ADP

الكواشف

لا شيء

المذيبات

O=P(O)(O)OC[C@H]1O[C@H](OP(=O)(O)OP(=O)(O)O)[C@H](O)[C@@H]1O
PRPP
O=P(O)(O)OC[C@H]1O[C@H](OP(=O)(O)OP(=O)(O)O)[C@H](O)[C@@H]1O
PRPP
O=P(O)(O)OC[C@H]1O[C@H](OP(=O)(O)OP(=O)(O)O)[C@H](O)[C@@H]1O
PRPP

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىThe following additions were made to each reaction
  2. 2
    أخرىThis second reaction
  3. 3
    workup.WAITto proceed at room temperature for 20 min

الإجراء التجريبي

The following additions were made to each reaction: 2 μl of PRPP, 2 μl of PRPP Synthetase, and 20 μl PRPP Synthetase buffer. The reactions proceeded at 37° C. for 28 minutes at which time the reactions were transferred to 100 μl containing 2 μl of ADP and 2 μl of NDPK. This second reaction was allowed to proceed at room temperature for 20 min. The amount of ATP produced was measured by the addition of 10 ng of Luciferase followed by measuring light output with a luminometer. The data is presented in Table 30.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US06379898B2uspto-grants-2002_04