تفاعل #8546

معادلة التفاعل

Methyl 2-(2,3,4-trifluoroanilino)propionate

methyl (2R)-2-(2,3,4-trifluoroanilino)propionate

→

title compound

المردود 47.9%

(2S)-2-(2,3,4-Trifluoroanilino)propionic acid

المردود 47.9%

المذيبات

phosphate

ظروف التفاعل

المعالجة

1. 1
   workup.ADDITIONThen the above-described liquid culture (10 ml) was added
2. 2
   workup.STIRRINGThe mixture was stirred for additional 16 hours

الإجراء التجريبي

Microbial cells (IFO-1575; Zygoascus hellenicus) were cultured in an MY medium (pH 6.0; 50 ml) at 30° C. for 48 hours. Methyl 2-(2,3,4-trifluoroanilino)propionate (1.0 g) was suspended in a 0.1 M phosphate buffer solution (pH 6.5; 90 ml). Then the above-described liquid culture (10 ml) was added thereto and gently stirred. The mixture was stirred for additional 16 hours while maintaining at 30° C. Then it was treated as in Example 43 to thereby give methyl (2R)-2-(2,3,4-trifluoroanilino)propionate (0.39 g, optical purity 91% ee) and the title compound (0.45 g, optical purity 84% ee).

المصدر