تفاعل #82860

ord-1825b608ebc244d3b221ee45bb66642a

معادلة التفاعل

CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]
DOTMA
CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC
DOPC
→
CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]
DOTMA DOPC

المتفاعلات

CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]
DOTMA
CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC
DOPC

الكواشف

لا شيء

المذيبات

ClC(Cl)Cl
chloroform

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىDOTMA/DOPC vesicles were prepared
  2. 2
    أخرىremoving the solvent
  3. 3
    أخرىby drying under a stream of nitrogen gas
  4. 4
    أخرىwas placed under vacuum overnight
  5. 5
    أخرىto remove traces of solvent
  6. 6
    أخرىsonicated in a bath type sonicator for 30 minutes

الإجراء التجريبي

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US05622712uspto-grants-1997_04