تفاعل #808138

معادلة التفاعل

water

N-acetyl-colchicinol

(2R,3R,4S,5R,6R)-2-[(5S)-5-(Acetylamino)-9,10,11-trimethoxy-6,7-dihydro-5H-dibenzo[a,c]cyclohepten-3-yl]oxy-3,5-bis(isobutyryloxy)-6-[(isobutyryloxy)methyl]tetrahydro-2H-pyran-4-yl 2-methylpropanoate

methyl dichlorophosphate

→

(5S)-5-(acetylamino)-9,10,11-trimethoxy-6,7-dihydro-5H-dibenzo[a,c]cyclohepten-3-yl methyl hydrogen phosphate

المردود 69.0%

المذيبات

THF

THF

THF

THF

ظروف التفاعل

المعالجة

1. 1
   workup.STIRRINGThe mixture was stirred at ambient temperature for 15 minutes
2. 2
   أخرىwas removed by evaporation
3. 3
   أخرىAfter removal of the insoluble material
4. 4
   ترشيحby filtration
5. 5
   أخرىthe filtrate was purified on HP20 SS resin
6. 6
   غسيلeluting with a gradient of 0–60% methanol/water
7. 7
   أخرىThe appropriate fractions were freeze-dried

الإجراء التجريبي

A solution of N-acetyl-colchicinol (1) (0.45 g; 1.26 mmol) in THF (40 ml) under argon was cooled to 0° C. and treated with a 1.0M solution of lithiumHMDS in THF (1.39 ml; 1.39 mmol). The mixture was stirred at 0° C. for 1 hour and then added in portions over about 15 minutes to a solution of methyl dichlorophosphate (625 μl; 4.16 mmol) in THF (150 ml). The mixture was stirred at ambient temperature for 15 minutes. After addition of water (200 ml) the THF was removed by evaporation. After removal of the insoluble material by filtration, the filtrate was purified on HP20 SS resin eluting with a gradient of 0–60% methanol/water. The appropriate fractions were freeze-dried to give (5S)-5-(acetylamino)-9,10,11-trimethoxy-6,7-dihydro-5H-dibenzo[a,c]cyclohepten-3-yl methyl hydrogen phosphate as white solid (391 mg).

المصدر