تفاعل #800719

ord-25ca403495bc4782b318fa2b4273bf7b

معادلة التفاعل

O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
[Cl-].[NH4+]
NH4Cl
CC1(C)S[C@@H]2[C@H](NC(=O)C(C(=O)O)c3ccccc3)C(=O)N2[C@H]1C(=O)O
carbenicillin
O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
Isopropyl-β-D-thiogalactopyranoside

المتفاعلات

—
[ 14 ]
O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
[Cl-].[NH4+]
NH4Cl
CC1(C)S[C@@H]2[C@H](NC(=O)C(C(=O)O)c3ccccc3)C(=O)N2[C@H]1C(=O)O
carbenicillin
O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

الكواشف

لا شيء

المذيبات

O=S(=O)(O)CCCN1CCOCC1
MOPS

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    workup.ADDITIONWhen appropriate, antibiotics were added in the following concentrations

الإجراء التجريبي

All liquid cultivations were conducted in MOPS minimal medium (Teknova) [14] cultures supplemented with 5 g/l glucose and an additional 4 g/l NH4Cl. When appropriate, antibiotics were added in the following concentrations: 100 μg/ml carbenicillin for the maintenance of pTrc- or pET-derived plasmids, 34 μg/ml chloramphenicol for pHACM-derived plasmids, 68 μg/ml chloramphenicol for pACYC-derived plasmids and pRARE2 (Novagen), and 20 μg/ml kanamycin for pACKm-derived plasmids. Isopropyl-β-D-thiogalactopyranoside (IPTG, EMD Chemicals) was provided at a concentration of 1 mM for the induction of expression from both trc and T7 promoters. Cultures for L-phenylalanine auxotrophic (ΔpheA) strains were additionally supplemented with L-phenylalanine (Sigma) at a concentration of 0.35 mM. For malonyl CoA availability experiments, cerulenin (Cayman Chemicals) and sodium malonate dibasic (Sigma) were added at a concentration 20 μg/ml and 2 g/l (1 g/l added twice), respectively.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US09181539B2uspto-grants-2015_11